INFECTIOUS DISEASES

Spectrum and Services :

Infectiousdiseases :

1) General ID – OP and IP consultation and admissions for Tropical Infectious diseases, Tuberculosis Fungal infections, Bacterial infections, Viral infections, Infections in people living with AIDS, Infections in immunocompromised patients, Infections in critical care units.
2) Transplant ID – OP and IP consultation and admissions for Haemopoetic Stem Cell Transplant (Bone Marrow Transplant) and Solid organ transplant patients – pre transplant work up and post-transplant follow up for transplant associated infections.
3) Travel clinic – OP consultation for vaccinations, health check-up, forms and certificates.
4) Paediatric ID – OP and IP consultation and admissions for paediatric infectious diseases, OP consultation for vaccination.

Microbiology laboratory – State-of-the-art facilities and automation for conventional and molecular laboratory diagnosis of common and rare bacterial, fungal, viral, parasitic infections; diagnosis of tuberculosis and tropical infections. Laboratory is accredited by NABL (National Accreditation Board for Testing and Calibrating Laboratories) since 2007.

Facilities :

1) OP consultation for infectious diseases daily and round the clock IP ID consultation.

2) Comprehensive diagnosis of sepsis – Automated blood culture systems, BD FX series from Becton Dickinson, USA. Capacity to process 720 blood culture samples at any given time.Rapid diagnosis of sepsis within 6 hrs of blood culture positivity using innovative tests like ‘Sepsityper’ and ‘Sepsis Flow’

Bactec FX blood culture

 

3) MALDI-TOF-MS – Highly expanded and rapid identification of bacteria, fungi, Mycobacteria, employing proteomic analysis of microorganisms using laser beam ionisation. CEID has the latest version of the Bruker-Biotyper Sirius MALDI system from Bruker Daltonics, Germany

Bruker-Biotyper Sirius MALDI-TOF-MS

 

4) Antibiotic sensitivity testing of variety of bacteria performed for multiple basic and high end antibiotics using a fully automated system BD Phoenix.

5) Immunological assays – Serological markers i.e. antigen and antibody testing for diagnosis of variety of infections like HIV, Hepatitis viruses, TORCH infections, C. difficile, tropical infections like Brucella, Salmonella, Dengue; biomarkers testing for CRP, procalcitonin.

6) Comprehensive diagnosis of tuberculosis – Culture of Mycobacterium tuberculosis and Non tuberculous Mycobacteria (NTM) on the BD MGIT 960 platform for diagnosis of pulmonary and extra pulmonary TB. Drug susceptibility testing to all 1st, 2nd and 3rd line anti-TB agents performed on MGIT. Drug susceptibility testing of NTM done by broth micro dilution. Rapid diagnosis of drug resistant tuberculous with 1 day of specimen receiving by detecting drug resistance genetic mutations on the Cepheid GeneXpert platform.

MGIT system for TB culture

 

7) Comprehensive diagnosis of invasive fungal infections – State-of-the-art mycology laboratory for culture isolation of common and rare fungi causing invasive fungal infections. Antifungal drug susceptibility testing performed using the gold standard broth micro dilution method. Facility for testing biomarkers for fungal infections like serum Galactomannan and serum Beta-D-glucan.

Mycology laboratory

 

8) Therapeutic drug monitoring – One of its kind facility in India for accurate estimation of plasma drug levels of various antimicrobial agents like Vancomycin, Teicoplanin, Linezolid, Rifampicin, Amikacin, Colistin, Voriconazole, Isavuconazole, Posaconazole using latest LCMS-MS technology.

LCMS-MS

 

9) Molecular diagnostics laboratory - State-of-the-art molecular laboratory with total automation for various technologies like PCR, Multiplex PCR, hybridization assays, FISH. Laboratory houses the DNA sequencing technology using Sanger and NGS (Next Generation Sequencing) platforms from ThermoFisher. 16 S rRNA Sequencing performed on various clinical samples using the ion semiconductor sequencing technology on the ThermoFisher Ion Torrent platform for diagnosis of bacterial infections due to non-cultivable or slow growing bacteria and microbiome studies.

NGS – Ion Torrent

 

Location of Department :

3rd floor, Vimal Mutha cancer centre building (Annex building) Deenanath Mangeshkar Hospital and Research Centre

Departmental Timetable :

MicrobiologyLaboratory–

• Functionalalldays,24hrs.
• Reportingtiming–9AMto5PM,MondaytoSaturday.Sundayemergencyreportingonly.

Departmental Workload :

InfectiousDisease

• OPDpatients–10000/year
• IPDconsultation–10000/year

Microbiology(includingmoleculartest) –

• Totalnumberoftests–150000tests/year

Courses and Training :

1) Post-doctoral fellowship in infectious Diseases and Microbiology commenced in 2021 2 admissions/ year, stipend paid
Program directors – Dr. Parikshit Prayag (ID) and Dr. Sampada Patwardhan (Microbiology)
2-year program – Basic and advanced course with regular didactics and hand on training
Presentation by fellows in various national and international conferences

2) DR CEID is one of the five centres in the country recognised for preceptorship for the ICCARe course for intensivists (ISCCM Course to Combat Antimicrobial Resistance). This includes didactic lectures and a one-day training on laboratory aspects of diagnosis of antibiotic resistance. The course is run yearly.

Events (since 2022):

1. Masterclass on gram negative infections – Jan-Feb 2022, Intensivists and Microbiologists from Pune – 8 weekend lectures (conducted by Dr, Parikshit Prayag and Dr. Sampada Patwardhan) plus one full day of in house training in the lab
2. Molecular workshop – 25th and 26th June 2022, 41 practicing microbiologists and post graduate students – lectures, demonstrations and hand-on training of various molecular diagnostic techniques
3. MALDI workshop – 16/10/2022, 20 microbiologists from the city of Pune – Lectures, demonstrations and hand-on training of MALDI-TIF MS techniques
4. Antimicrobial Stewardship workshop – 2/3/4 February 2023, participation from more than 14 hospitals across Pune and Maharashtra, with more than 150 delegates – role of antimicrobial stewardship in bacterial, fungal infections, role of laboratory, surgical prophylaxis. SWOT analysis exercises.
5. MycoCon 2023 pre-conference workshop – National fungal conference (conducted by the Fungal infections Study Forum of India), 8th September 2023, 65 delegates- Live demos and lectures on conventional morphological methods of fungal diagnosis, antifungal susceptibility testing, MALDI-TOF-MS, biomarker tests.

International collaborations – Exchange of fellowship program and educational activities, Joint organization of training workshops and conferences, Joint research activities

1) Henry Ford Hospital (HFH), Detroit, USA

2) Keck School of Medicine, University of South California

Outreach – For upgrading diagnostic infrastructure, training of technician and microbiologists, ID consultation services, outreach for training of intensivists, physicians and microbiologists

1) Hedgewar hospital, Aurangabad

2) Vivekanand hospital, Latur

3) Christian Medical College Ludhiana, Punjab

4) Bangabandhu Sheikh Mujibur Rahman University, Bangladesh

Research –

CEID has participated in the past and has currently ongoing numerous clinical trials for new antibiotic molecules. Faculty in the department has contributed several research publications in national and international Infectious Diseases and Clinical Microbiology journals.

Other activities –

The department leads the hospital infection control program by performing surveillance activities, conducting audits, formulating antimicrobial policy for the hospital, conducting educational activities related to nosocomial infections and infection control.